The ZEISS Lattice Lightsheet 7 is a specialized system designed for long-term, high-speed 3D imaging of living specimens. It utilizes a patented inverted optical geometry that allows researchers to use standard sample carriers such as multi-well plates, Petri dishes, and coverslips, bypassing the complex mounting requirements of traditional light-sheet systems. By employing an extremely thin lattice light-sheet, the system illuminates only the focal plane, which significantly reduces photobleaching and cellular stress. This minimal phototoxicity, combined with high volumetric speed and stable environmental control, makes it ideal for capturing rapid biological processes in 3D over several hours or days. Typical applications include for example tracking subcellular organelle interactions, observing early-stage embryo development, and studying cell migration or cancer cell invasion within 3D collagen matrices.
We are grateful for the funding support provided by France BioImaging via the FranceInnovation2030 call and La Ligue contre le cancer; this system is open to the France BioImaging community.
